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Isolation of Babesia bigemina and Babesia bovis merozoites by ammonium chloride lysis of infected erythrocytes
Braz. j. med. biol. res ; 27(11): 2591-8, Nov. 1994. ilus
Article in En | LILACS | ID: lil-153980
Responsible library: BR1.1
ABSTRACT
1. We describe the isolation of viable merozoites from erythrocytes infected with Babesia bovis or Babesia bigemina organisms by ammonium chloride lysis. 2. Parasite morphology was examined by both light and transmission electron microscopy. Erythrocyte-free parasites maintain their viability and infectivity, retain their antigenicity and are suitable for use in the indirect fluorescent antibody assay
Subject(s)
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Index: LILACS Main subject: Babesia / Babesia bovis / Erythrocytes Limits: Animals Language: En Journal: Braz. j. med. biol. res Journal subject: BIOLOGIA / MEDICINA Year: 1994 Type: Article
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Index: LILACS Main subject: Babesia / Babesia bovis / Erythrocytes Limits: Animals Language: En Journal: Braz. j. med. biol. res Journal subject: BIOLOGIA / MEDICINA Year: 1994 Type: Article