Evaluation of a direct immunofluorescent antibody (Difma) test using Leishmania genus specific monoclonal antibody in the routine diagnosis of cutaneous leishmaniasis
Rev. Soc. Bras. Med. Trop
; 28(2): 99-103, abr.-jun. 1995. tab
Article
in En
| LILACS
| ID: lil-163751
Responsible library:
BR1.1
ABSTRACT
A direct immunofluorescent antibody (DIFMA) test using a Leishmania genus-specific monoclonal antibody was evaluated in the routine diagnosis of cutaneous leishmaniasis (CL) in Ecuador. This test was compared with the standard diagnostic techniques of scrapings, culture and histology. Diagnostic samples were taken from a total of 90 active dermal ulcers from patients from areas of Ecuador known to be endemic for cutaneous leishmaniasis. DIFMA was positive in all lesions. It was shown to by significantly superior to standard diagnostic methods either alone or in combination. The sensitivity of DIFMA did not diminish with chronicity of lesions. This test proved to be extremely useful in the routine diagnosis of CL because it is highly sensitive, is easy to use and produces rapid results.
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Index:
LILACS
Main subject:
Antibodies, Protozoan
/
Leishmaniasis, Cutaneous
/
Leishmania
/
Antibodies, Monoclonal
/
Antibody Specificity
Type of study:
Diagnostic_studies
Limits:
Animals
/
Female
/
Humans
/
Male
Country/Region as subject:
America do sul
/
Ecuador
Language:
En
Journal:
Rev. Soc. Bras. Med. Trop
Journal subject:
MEDICINA TROPICAL
Year:
1995
Type:
Article