Diameter of the mammalian porin channel in open and "closed" states: direct measurement at the single channel level in planar lipid bilayer
Braz. j. med. biol. res
; 29(12): 1691-7, Dec. 1996. graf
Article
in En
| LILACS
| ID: lil-188456
Responsible library:
BR1.1
RESUMO
Porin isolated from bovine skeletal muscle was reconstituted in planar lipid bilayers under voltage clamp conditions. A set of non-electrolytes were used as molecular probes for determining the pore diameter. The maximal diameter of the open channel was estimated to be 3.02 + 0.26 nm. As observed for other porin channels, a large transmembrane potential drove the channel into a "closed" state. The channel transition to the low conductance (closed) state was followed by a decrease in the maximal diameter of the channel to 2.4 +- 0.08 nm.
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Index:
LILACS
Main subject:
In Vitro Techniques
/
Porins
/
Ion Channels
/
Lipid Bilayers
/
Mammals
Limits:
Animals
Language:
En
Journal:
Braz. j. med. biol. res
Journal subject:
BIOLOGIA
/
MEDICINA
Year:
1996
Type:
Article