Comparison of the effect of hypothalamic neuropeptides upon luteinizing hormone secretion by cultured rat anterior pituitary cells
Biol. Res
;
27(2): 113-21, 1994. graf
Article
in English
| LILACS
| ID: lil-226225
ABSTRACT
Bovine median eminence contains a factor difference from gonadotropin-releasing hormone (GnRH) than increases basal luteinizing hormone (LH) secretion and potentiates GnRH-stimulated LH release. We compared the effect of hypothalamic neuropeptides on basal and GnRH-stimulated LH secretion using rat pituitary cells under static incubation conditions to determine if any of them mimics the LH-releasing activity no attributable to GnRH present in bovine median eminence extracts. Both, galanin and eurotensin (10(-9)-10(-5)) stimulated basal LH secretion in a dose-response manner. Galaninincreased 3-4 fold and neurotensin doubled the basal LH secretion. The GnRH antagonist Nal-Glu 10(-6) M abolished the effect of 10(-7) M GnRH and 10(-5)M neurotensin, but did not block the LH-releasing activity of galanin. Leucin-enkephalin, beta-endorphin, substance P and neuropeptide Y (NPY) did not alter basal LH secretion. Neuropeptides produced three types of response on GnRH-stimulated LH release. First...
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Index:
LILACS (Americas)
Main subject:
Neuropeptides
/
Luteinizing Hormone
/
Gonadotropin-Releasing Hormone
/
Gonadotropins, Pituitary
Limits:
Animals
Language:
English
Journal:
Biol. Res
Journal subject:
Biology
Year:
1994
Type:
Article
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