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Enzyme-linked immunosorbent assay for detection and quantification of Cryptococcus neoformans antigen
Illnait, Ma. Teresa; Vilaseca, Juan C; Fernández, Carlos M; Martínez, Gerardo F.
Affiliation
  • Illnait, Ma. Teresa; Instituto Pedro Kourí. Laboratorio de Micología. Havana. CU
  • Vilaseca, Juan C; Instituto Pedro Kourí. Laboratorio de Micología. Havana. CU
  • Fernández, Carlos M; Instituto Pedro Kourí. Laboratorio de Micología. Havana. CU
  • Martínez, Gerardo F; Instituto Pedro Kourí. Laboratorio de Micología. Havana. CU
Mem. Inst. Oswaldo Cruz ; 96(2): 241-5, Feb. 2001. ilus
Article in En | LILACS | ID: lil-281574
Responsible library: BR1.1
ABSTRACT
An enzyme-linked immunosorbent assay was standardized for the detection of cryptococcal antigen in serum and cerebrospinal fluid. The system was evaluated in clinical samples from patients infected by human immunodeficiency virus with and without previous cryptococcosis diagnosis. The evaluated system is highly sensitive and specific, and when it was compared with latex agglutination there were not significant differences. A standard curve with purified Cryptococcus neoformans antigen was settled down for the antigen quantification in positive samples
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Full text: 1 Index: LILACS Main subject: Polysaccharides / Acquired Immunodeficiency Syndrome / Cryptococcus neoformans / Antigens, Fungal Type of study: Diagnostic_studies Limits: Humans Language: En Journal: Mem. Inst. Oswaldo Cruz Journal subject: MEDICINA TROPICAL / PARASITOLOGIA Year: 2001 Type: Article
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Full text: 1 Index: LILACS Main subject: Polysaccharides / Acquired Immunodeficiency Syndrome / Cryptococcus neoformans / Antigens, Fungal Type of study: Diagnostic_studies Limits: Humans Language: En Journal: Mem. Inst. Oswaldo Cruz Journal subject: MEDICINA TROPICAL / PARASITOLOGIA Year: 2001 Type: Article