Resistance of Biomphalaria occidentalis from Varzea das Flores dam, Minas Gerais, to Schistosoma mansoni infection detected by low stringency polymerase chain reaction
Mem. Inst. Oswaldo Cruz
; 96(3): 381-383, Apr. 2001. ilus, tab
Article
in En
| LILACS
| ID: lil-282849
Responsible library:
BR1.1
RESUMO
Biomphalaria occidentalis Paraense, 1981 from Varzea das Flores dam, MG, Brazil, was exposed to infection with Schistosoma mansoni. Individual infection was performed with 140 B. occidentalis and 100 B. glabrata snails using LE and SJ strains. Two groups of B. occidentalis were killed after seven day-miracidia exposure to detect S. mansoni DNA, through the low stringency polymerase chain reaction (LS-PCR), and were negative. The infection rates were 69.2 percent (LE strain) and 96.7 percent (SJ strain) for B. glabrata and 0 percent for B. occidentalis. LS-PCR enabled early resistance diagnosis
Full text:
1
Index:
LILACS
Main subject:
Schistosoma mansoni
/
Biomphalaria
/
Schistosomiasis mansoni
/
Polymerase Chain Reaction
/
Host-Parasite Interactions
Limits:
Animals
Language:
En
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
MEDICINA TROPICAL
/
PARASITOLOGIA
Year:
2001
Type:
Article