Nascimento de bezerra gerada com auxílio das técnicas de punçäo folicular e fertilizaçäo in vitro no Estado de Minas Gerais / Birth of a calf produced by follicular puncture and in vitro fertilization techniques in Minas Gerais State, Brazil

RESUMO

Ultrasound-assisted follicular puncture is a technique of oocyte recovery for in vitro fertilization and production of embryos. In order to adjust the methodology, a field trial was performed using lactating Holstein cows (n=8) as oocyte donors. Punctures were done with an ultrasound device equipped with a 7.5 MHz intravaginal sectorial probe. Oocytes recovered were matured, fertilized and cultured in vitro, and embryos produced transferred to recipients heifers. On July, 2000, the first calf produced by these techniques in Minas Gerais State, Brazil, was born