Mtp-40 and alpha antigen gene fragment amplification for the detection of Mycobacterium tuberculosis in Colombian clinical specimens
Mem. Inst. Oswaldo Cruz
; 97(8): 1157-1163, Dec. 15, 2002. ilus, tab
Article
in En
| LILACS
| ID: lil-326328
Responsible library:
BR1.1
RESUMO
In this study, the use of Mtp-40 and alpha antigen polymerase chain reaction (PCR) amplification fragments for the precise tuberculosis (TB) diagnosis was evaluated. One hundred and ninety two different samples were obtained from 113 patients with suspected TB. Mtp-40 and alpha antigen protein genes were amplified by the PCR technique and compared to both the "gold standard" (culture) test, as well as the clinical parameters (including a clinical record and X-ray film exam in 113 patients). Thirty-eight of the 113 patients had a presumptive clinical diagnosis of TB; 74 percent being detected by PCR technique, 58 percent by culture and 44 percent by direct microscopic visualization. Weconclude that it is possible to use PCR as a suitable technique for the detection of any mycobacteria by means of the alpha antigen product, or the specific infection of Mycobacterium tuberculosis by means of the mtp-40 gene. This might be a good supporting tool in difficult clinical TB diagnosis and pauci-bacillary cases
Full text:
1
Index:
LILACS
Main subject:
Bacterial Proteins
/
Tuberculosis
/
Mycobacterium tuberculosis
/
Antigens, Bacterial
Type of study:
Diagnostic_studies
/
Evaluation_studies
Limits:
Humans
Country/Region as subject:
America do sul
/
Colombia
Language:
En
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
MEDICINA TROPICAL
/
PARASITOLOGIA
Year:
2002
Type:
Article