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Kinetics of T cell-activation molecules in response to Mycobacterium tuberculosis antigens
Antas, Paulo R. Z; Oliveira, Eliane B; Milagres, Alexandre S; Franken, Kees C; Ottenhoff, Tom H. M; Klatser, Paul; Sarno, Euzenir N; Sampaio, Elizabeth P.
Affiliation
  • Antas, Paulo R. Z; Fiocruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro. BR
  • Oliveira, Eliane B; Fiocruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro. BR
  • Milagres, Alexandre S; Hospital Municipal Raphael de Paula e Souza. Rio de Janeiro. BR
  • Franken, Kees C; Leiden University Medical Center. Department of Immunohematology and Blood Transfusion. Leiden. NL
  • Ottenhoff, Tom H. M; Leiden University Medical Center. Department of Immunohematology and Blood Transfusion. Leiden. NL
  • Klatser, Paul; Royal Tropical Institute. Department of Biomedical Research. Amsterdam. NL
  • Sarno, Euzenir N; Fiocruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro. BR
  • Sampaio, Elizabeth P; Fiocruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro. BR
Mem. Inst. Oswaldo Cruz ; 97(8): 1097-1099, Dec. 15, 2002. tab, graf
Article in En | LILACS | ID: lil-326339
Responsible library: BR1.1
RESUMO
The phenotypic features acquired subsequent to antigen-specific stimulation in vitro were evaluated by means of the kinetic expressions of CD69 and CD25 activation molecules on T lymphocytes and assayed by flow cytometry in response to PPD, Ag85B, and ferritin in PPD-positive healthy control individuals. In response to PHA, CD69 staining on both CD4+ and CD8+ T cells became initially marked after 4 h, peaked at 24 h, and quickly decreased after 120 h. For CD25, a latter expression was detected around 8 h, having increased after 96 h. As expected, the response rate to the mycobacterial antigens was much lower than that to the mitogen. Positive staining was high after 96 h for CD25 and after 24 h for CD69. CD69 expression was significantly enhanced (p < 0.05) on CD8+ as compared to CD4+ T cells. High levels were also found between 96-120 h. Regarding Ag85B, CD25+ cells were mostly CD4+ instead of CD8+ T cells. Moreover, in response to ferritin, a lower CD25 expression was noted. The present data will allow further characterization of the immune response to new mycobacterial-specific antigens and their evaluation for possible inclusion in developing new diagnostic techniques for tuberculosis as well in a new vaccine to prevent the disease
Subject(s)
Full text: 1 Index: LILACS Main subject: Lymphocyte Activation / CD4-Positive T-Lymphocytes / Receptors, Interleukin-2 / Antigens, Differentiation, T-Lymphocyte / Antigens, CD / CD8-Positive T-Lymphocytes / Mycobacterium tuberculosis / Antigens, Bacterial Language: En Journal: Mem. Inst. Oswaldo Cruz Journal subject: MEDICINA TROPICAL / PARASITOLOGIA Year: 2002 Type: Article
Full text: 1 Index: LILACS Main subject: Lymphocyte Activation / CD4-Positive T-Lymphocytes / Receptors, Interleukin-2 / Antigens, Differentiation, T-Lymphocyte / Antigens, CD / CD8-Positive T-Lymphocytes / Mycobacterium tuberculosis / Antigens, Bacterial Language: En Journal: Mem. Inst. Oswaldo Cruz Journal subject: MEDICINA TROPICAL / PARASITOLOGIA Year: 2002 Type: Article