Protocol for extraction of genomic DNA from swine solid tissues
Genet. mol. biol
; 25(3): 313-315, Sept. 2002. ilus, tab
Article
in En
| LILACS
| ID: lil-335773
Responsible library:
BR26.1
ABSTRACT
Molecular diagnostics are performed by using DNA from different body tissues. However, it is necessary to obtain genomic DNA of good quality. Due to the impossibility of collecting blood from slaughtered animals, DNA extraction from solid tissues is necessary. The objective of this study was to describe a protocol of DNA extraction from swine skin, adipose, brain, liver, kidney and muscle tissues. We obtained high molecular weight DNA of good quality, shown by agarose gel and amplification of two DNA fragments, 605bp and 891pb, by PCR. Spectrophotometric analysis of DNA concentration showed variation among the DNA from different tissues, with the liver and adipose tissues presenting the greatest and the smallest concentration, respectively. The described protocol has proven to be advantageous due to its simplicity, quickness, affordable reagents and absence of phenol, resulting in a high molecular weight DNA of good quality from several tissues
Full text:
1
Index:
LILACS
Main subject:
Swine
/
DNA
/
Polymerase Chain Reaction
Type of study:
Guideline
Limits:
Animals
Language:
En
Journal:
Genet. mol. biol
Journal subject:
GENETICA
Year:
2002
Type:
Article