Mutación del gen K-ras en el cáncer de la vesícula biliar / K-ras gene mutation in gallbladder carcinoma
Rev. méd. Chile
;
132(8): 955-960, ago. 2004. ilus, tab
Article
in Spanish
| LILACS
| ID: lil-384191
RESUMO
Background:
Different K-ras mutation frequencies in gallbladder cancer have been reported.Aim:
To study the frequency of K-ras gene mutations in advanced gallbladder carcinoma not associated to anomalous junction of pancreatic-biliary duct (AJPBD). Material andmethods:
33 formalin fixed paraffin embedded samples of gallbladder carcinoma (30 women, age range 32-86 years) were selected. Pancreatic cancer tissue with K-ras mutations was used as control. DNA was extracted from the histological section by mean of microdissection and K-ras mutations in codon 12 were detected by polymerase chain reaction and restriction fragment length polymorphism (RFLP), using previously reported technique.Results:
Most cases were poorly differentiated adenocarcinomas. K-ras mutation was detected in 10 cases (30%) samples. No differences in K-ras mutation frequency between subserous and serous tumors were detected and no relation between histological features and the mutation was observed.Conclusions:
K-ras mutation in codon 12 is present in 30% in our advanced gallbladder carcinomas. The study of K-ras mutation in preneoplastic lesions and early carcinomas will be important to determine the role of this gene in the gallbladder carcinogenesis in Chile (Rev Méd Chile 2004; 132 955-60).
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Adenocarcinoma
/
Gallbladder Neoplasms
/
Mutation
Limits:
Adult
/
Female
/
Humans
/
Male
Language:
Spanish
Journal:
Rev. méd. Chile
Journal subject:
Medicine
Year:
2004
Type:
Article
/
Project document
Affiliation country:
Chile
Institution/Affiliation country:
Universidad de La Frontera/CL
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