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HygR and PurR plasmid vectors for episomal transfection of Trypanosoma cruzi
Nóbrega, Otávio T; Santana, Jaime M; Sturm, Nancy R; Teixeira, Antônio R. L; Campbell, David A.
  • Nóbrega, Otávio T; Universidade de Brasília. Faculdade de Medicina. Laboratório Multidisciplinar de Pesquisa em Doença de Chagas. Brasília. BR
  • Santana, Jaime M; Universidade de Brasília. Faculdade de Medicina. Laboratório Multidisciplinar de Pesquisa em Doença de Chagas. Brasília. BR
  • Sturm, Nancy R; University of California. David Geffen School of Medicine. Department of Microbiology, Immunology and Molecular Biology. Los Angeles. US
  • Teixeira, Antônio R. L; Universidade de Brasília. Faculdade de Medicina. Laboratório Multidisciplinar de Pesquisa em Doença de Chagas. Brasília. BR
  • Campbell, David A; University of California. David Geffen School of Medicine. Department of Microbiology, Immunology and Molecular Biology. Los Angeles. US
Mem. Inst. Oswaldo Cruz ; 99(5): 513-516, Aug. 2004. ilus
Article in English | LILACS | ID: lil-386684
RESUMO
This work describes the development and functional testing of two episomes for stable transfection of Trypanosoma cruzi. pHygD contained the 5'- and 3'- flanking regions of the gene encoding the cathepsin B-like protease of T. cruzi as functional trans-splicing and polyadenylation signals for the hygR ORF. Evidence is presented to support extrachromosomal maintenance and organization as tandem repeats in transfected parasites. pPac was derived from pHygD by replacement of the entire hygR ORF with a purR coding region. The ability to modify pHygD and the availability of the complete DNA sequence make these plasmids useful tools for the genetic manipulation of T. cruzi.
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Full text: Available Index: LILACS (Americas) Main subject: Trypanosoma cruzi / Transfection / Genetic Vectors Limits: Animals Language: English Journal: Mem. Inst. Oswaldo Cruz Journal subject: Tropical Medicine / Parasitology Year: 2004 Type: Article / Project document Affiliation country: Brazil / United States Institution/Affiliation country: Universidade de Brasília/BR / University of California/US

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Full text: Available Index: LILACS (Americas) Main subject: Trypanosoma cruzi / Transfection / Genetic Vectors Limits: Animals Language: English Journal: Mem. Inst. Oswaldo Cruz Journal subject: Tropical Medicine / Parasitology Year: 2004 Type: Article / Project document Affiliation country: Brazil / United States Institution/Affiliation country: Universidade de Brasília/BR / University of California/US