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Detection of Brazilian spotted fever infection by polymerase chain reaction in a patient from the state of São Paulo
Nascimento, Elvira Maria Mendes; Gehrke, Flávia de Sousa; Maldonado, Rosa Amélia; Colombo, Silvia; Silva, Luiz Jacintho da; Schumaker, Teresinha Tizu Sato.
Affiliation
  • Nascimento, Elvira Maria Mendes; Instituto Adolfo Lutz. Laboratório de Rickettsias. São Paulo. BR
  • Gehrke, Flávia de Sousa; Universidade de São Paulo. Instituto de Ciências Biomédicas. Departamento de Parasitologia. São Paulo. BR
  • Maldonado, Rosa Amélia; University of Texas at EL Paso. Dept. of Biological Sciences. US
  • Colombo, Silvia; Instituto Adolfo Lutz. Laboratório de Rickettsias. São Paulo. BR
  • Silva, Luiz Jacintho da; Universidade Estadual de Campinas. Faculdade de Ciências Médicas. Departamento de Clínica Médica. São Paulo. BR
  • Schumaker, Teresinha Tizu Sato; Universidade de São Paulo. Instituto de Ciências Biomédicas. Departamento de Parasitologia. São Paulo. BR
Mem. Inst. Oswaldo Cruz ; 100(3): 277-279, May 2005. ilus
Article in En | LILACS, SES-SP | ID: lil-411024
Responsible library: BR1.1
ABSTRACT
Brazilian spotted fever (BSF) cases have been increasing in the state of São Paulo but no genomic information about local rickettsia isolated from humans has been well documented. We recovered spotted-fever group rickettsiae from a sample of patient blood cultured in Vero cells using the shell vial technique. Rickettsial DNA fragments (gltA, ompA, and, ompB genes) were detected, and analysis of the ompB gene base sequences showed identity with the Rickettsia rickettsii ompB sequence available in the GenBank.
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Full text: 1 Index: LILACS Main subject: Rickettsia rickettsii / Rickettsia Infections / Bacterial Outer Membrane Proteins / DNA, Bacterial Type of study: Diagnostic_studies Limits: Animals / Humans / Male Country/Region as subject: America do sul / Brasil Language: En Journal: Mem. Inst. Oswaldo Cruz Year: 2005 Type: Article
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Full text: 1 Index: LILACS Main subject: Rickettsia rickettsii / Rickettsia Infections / Bacterial Outer Membrane Proteins / DNA, Bacterial Type of study: Diagnostic_studies Limits: Animals / Humans / Male Country/Region as subject: America do sul / Brasil Language: En Journal: Mem. Inst. Oswaldo Cruz Year: 2005 Type: Article