Evaluation of the dot enzyme-linked immunosorbent assay in comparison with standard ELISA for the immunodiagnosis of human toxocariasis
Mem. Inst. Oswaldo Cruz
; 101(1): 71-74, Feb. 2006.
Article
in En
| LILACS
| ID: lil-423565
Responsible library:
BR1.1
ABSTRACT
A dot enzyme-linked immunosorbent assay (dot-ELISA) was standardized using excretory-secretory antigens of Toxocara canis for the rapid immunodiagnosis of human toxocariasis. Thirty patients with clinical signs of toxocariasis, 20 cases with other parasitic diseases, and 40 healthy subjects were tested. A total of 0.2 ng of antigen per dot, serum dilution of 1160 and dilution conjugate of 11000 were found optimal. The sensitivity and specificity of the assay were 100 and 95 percent, respectively. Comparable sensitivity of dot-ELISA and the standard ELISA was obtained, but only 3 cross-reactions occurred in the dot-ELISA, compared with 6 in the standard ELISA. Dot-ELISA is simple to perform, rapid, and low cost. Large-scale screening studies should be done to evaluate its usefulness under field conditions.
Full text:
1
Index:
LILACS
Main subject:
Enzyme-Linked Immunosorbent Assay
/
Antibodies, Helminth
/
Toxocariasis
Type of study:
Diagnostic_studies
/
Observational_studies
/
Prognostic_studies
/
Risk_factors_studies
Limits:
Animals
/
Humans
Language:
En
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
MEDICINA TROPICAL
/
PARASITOLOGIA
Year:
2006
Type:
Article