Giardia duodenalis: analysis of secreted proteases upon trophozoite-epithelial cell interaction in vitro
Mem. Inst. Oswaldo Cruz
; 101(6): 693-696, Sept. 2006. ilus, tab, graf
Article
in En
| LILACS
| ID: lil-437067
Responsible library:
BR1.1
ABSTRACT
Protease secretion by Giardia duodenalis trophozoites upon interaction with epithelial cells and its association with the parasite adhesion was studied in co-cultures of parasites with IEC6 epithelial cell monolayers in the presence or absence of protease inhibitors. Proteolytic activity in supernatants from trophozoites was enhanced when they were co-cultured with IEC6 cells. This activity was strongly inhibited by pre-incubation of live trophozoites with E-64 and TPCK and a concomitant inhibition of parasite adhesion to IEC6 cells was observed. These data suggest that trophozoites secrete cysteine-type proteases that play a role in the adhesion of G. duodenalis to epithelial cells.
Full text:
1
Index:
LILACS
Main subject:
Peptide Hydrolases
/
Protease Inhibitors
/
Epithelial Cells
/
Giardia
Limits:
Animals
Language:
En
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
MEDICINA TROPICAL
/
PARASITOLOGIA
Year:
2006
Type:
Article