Antimalarial drugs disrupt ion homeostasis in malarial parasites
Mem. Inst. Oswaldo Cruz
;
102(3): 329-334, June 2007. graf
Article
in English
| LILACS
| ID: lil-452510
ABSTRACT
Plasmodium chabaudi malaria parasite organelles are major elements for ion homeostasis and cellular signaling and also target for antimalarial drugs. By using confocal imaging of intraerythrocytic parasites we demonstrated that the dye acridine orange (AO) is accumulated into P. chabaudi subcellular compartments. The AO could be released from the parasite organelles by collapsing the pH gradient with the K+/H+ ionophore nigericin (20 µM), or by inhibiting the H+-pump with bafilomycin (4 µM). Similarly, in isolated parasites loaded with calcium indicator Fluo 3-AM, bafilomycin caused calcium mobilization of the acidic calcium pool that could also be release with nigericin. Interestingly after complete release of the acidic compartments, addition of thapsigargin at 10 µM was still effective in releasing parasite intracellular calcium stores in parasites at trophozoite stage. The addition of antimalarial drugs chloroquine and artemisinin resulted in AO release from acidic compartments and also affected maintenance of calcium in ER store by using different drug concentrations.
Full text:
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Index:
LILACS (Americas)
Main subject:
Plasmodium chabaudi
/
Erythrocytes
/
Homeostasis
/
Ion Channels
/
Antimalarials
Limits:
Animals
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
Tropical Medicine
/
Parasitology
Year:
2007
Type:
Article
Affiliation country:
Brazil
/
United States
Institution/Affiliation country:
Universidade de São Paulo/BR
/
University of Medicine and Dentistry of New Jersey/US
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