Isolation and identification of bovine tuberculosis in a Brazilian herd (São Paulo)
Mem. Inst. Oswaldo Cruz
; 102(5): 639-642, Aug. 2007. tab
Article
in En
| LILACS
| ID: lil-458627
Responsible library:
BR1.1
ABSTRACT
Mycobacterium was verified in animals from a Brazilian dairy herd, a total of 42 samples from 30 cows were submitted to culture and the isolated strains were analyzed by two polymerase chain reaction (PCR), the first specific for species belonging to the Mycobacterium complex (MTBC) and the other for differentiating M. tuberculosis from M. bovis. Twenty seven samples (64.3 percent) from 18 animals (60 percent) were positive for mycobacteria by culture, including samples from 15 retrofaryngeal lymphnodes (55.5 percent), 9 prescapular lymphnodes (33.3 percent), 2 lungs (7.4 percent), and 1 liver (3.7 percent). All isolated colonies were confirmed by PCR to contain MTBC organisms, and were identified as M. bovis by the same methodology.
Key words
Full text:
1
Index:
LILACS
Main subject:
Tuberculosis, Bovine
/
Mycobacterium bovis
/
Mycobacterium tuberculosis
Type of study:
Diagnostic_studies
Limits:
Animals
Country/Region as subject:
America do sul
/
Brasil
Language:
En
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
MEDICINA TROPICAL
/
PARASITOLOGIA
Year:
2007
Type:
Article