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Multiplex-PCR serotyping of Listeria monocytogenes isolated from human clinical specimens
Vasconcelos, Rafaela Moledo de; Almeida, Antonio Eugenio Castro Cardoso de; Hofer, Ernesto; Silva, Nicéa Magaly Matias da; Marin, Victor Augustus.
Affiliation
  • Vasconcelos, Rafaela Moledo de; FIOCRUZ. Instituto Nacional de Controle e Qualidade em Saúde. Programa de Pós-Graduação em Vigilância Sanitária. Rio de Janeiro. BR
  • Almeida, Antonio Eugenio Castro Cardoso de; FIOCRUZ. Instituto Nacional de Controle e Qualidade em Saúde. Departamento de Microbiologia. Rio de Janeiro. BR
  • Hofer, Ernesto; FIOCRUZ. Instituto Nacional de Controle e Qualidade em Saúde. Laboratório de Zoonoses Bacterianas. Rio de Janeiro. BR
  • Silva, Nicéa Magaly Matias da; Clínica Perinatal de Laranjeiras. Rio de Janeiro. BR
  • Marin, Victor Augustus; FIOCRUZ. Instituto Nacional de Controle e Qualidade em Saúde. Departamento de Microbiologia. Rio de Janeiro. BR
Mem. Inst. Oswaldo Cruz ; 103(8): 836-838, Dec. 2008. ilus
Article in En | LILACS | ID: lil-502306
Responsible library: BR1.1
ABSTRACT
The genus Listeria is composed of six species of which Listeria monocytogenes is considered the single pathogenic species that causes listeriosis in humans. Of the 13 serovars of L. monocytogenes, 1/2a, 1/2b and 4b are responsible for the majority of clinical cases. The aim of this work was to detect L. monocytogenes in the cerebrospinal fluid sample of premature newborns and to characterize this sample using biotyping, serotyping and molecular typing. The results indicated the presence of L. monocytogenesin the clinical sample studied. Moreover, the isolate was identified as the 4b serovar that was characterized by the presence of a unique 691 bp band after analysis using the Multiplex-PCR technique. The results of repeated Multiplex-PCR and sequencing have indicated that the L. monocytogenes isolate was an atypical 4b serovar, which is the first time this finding has been reported.
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Full text: 1 Index: LILACS Main subject: Polymerase Chain Reaction / Listeriosis / Listeria monocytogenes Type of study: Prognostic_studies Limits: Humans / Newborn Language: En Journal: Mem. Inst. Oswaldo Cruz Journal subject: MEDICINA TROPICAL / PARASITOLOGIA Year: 2008 Type: Article
Full text: 1 Index: LILACS Main subject: Polymerase Chain Reaction / Listeriosis / Listeria monocytogenes Type of study: Prognostic_studies Limits: Humans / Newborn Language: En Journal: Mem. Inst. Oswaldo Cruz Journal subject: MEDICINA TROPICAL / PARASITOLOGIA Year: 2008 Type: Article