Ribotyping, biotyping and capsular typing of Haemophilus influenzae strains isolated from patients in Campinas, southeast Brazil
Braz. j. infect. dis
; 12(5): 430-437, Oct. 2008. tab, ilus
Article
in En
| LILACS
| ID: lil-505358
Responsible library:
BR1.1
ABSTRACT
Forty-five Haemophilus influenzae strains isolated from patients were characterized based on biochemical characteristics. Their capsular types were determined by polymerase chain reaction (PCR); they were compared, using two molecular methods [ribotyping with a specific DNA probe amplified from the 16S rDNA region from H. influenzae and through restriction fragment length polymorphism (RLFP) of an amplified 16S DNA region]. The strains were better discriminated by the ribotyping technique that used the 16S probe and by the combination of both techniques. Biotypes I and IV were the most common, followed by biotypes VI, VIII and III. Biotypes II and VII were not found. Most of the capsular samples were nontypable (89 percent), with capsular types a and b found in 2 and 9 percent of the samples, respectively. We concluded that there is a very close genetic identity among pathogenic and non-pathogenic strains.
Key words
Full text:
1
Index:
LILACS
Main subject:
DNA, Bacterial
/
DNA, Ribosomal
/
Haemophilus influenzae
/
Bacterial Typing Techniques
Limits:
Humans
Country/Region as subject:
America do sul
/
Brasil
Language:
En
Journal:
Braz. j. infect. dis
Journal subject:
DOENCAS TRANSMISSIVEIS
Year:
2008
Type:
Article
/
Project document