Screening for ligninolytic enzymes from autochthonous fungi and applications for decolorization of Remazole Marine Blue / Triagem de enzimas ligninolíticas de fungos autóctones e aplicações para descoloramento de Remazole Marine Blue
Braz. j. microbiol
; Braz. j. microbiol;40(2): 346-353, Apr.-June 2009. ilus, graf, tab
Article
in En
| LILACS
| ID: lil-520222
Responsible library:
BR32.1
ABSTRACT
This study presents new and alternative fungal strains for the production of ligninolytic enzymes which have great potential to use in industrial and biotechnological processes. Thirty autochthonous fungal strains were harvested from Bornova-Izmir in Turkiye. In the fresh fruitbody extracts laccase, manganese peroxidase and lignin peroxidase activities, which are the principal enzymes responsible for ligninocellulose degradation by Basidiomycetes, were screened. Spores of some of the basidiomycetes species such as Cortinarius sp., Trametes versicolor, Pleurotus ostreatus, Abortiporus biennis, Lyophyllum subglobisporium, Ramaria stricta, Ganoderma carnosum, Lactarius delicious ve Lepista nuda were isolated and investigated optimum cultivation conditions in submerged fermentation for high yields of ligninolytic enzyme production. In addition, isolated fungal strains were monitored on agar plates whether having the capability of decolorization of a textile dye Remazol Marine Blue.
RESUMO
Este estudo apresenta novas cepas de fungos produtores de enzimas ligninolíticas com potencial de aplicação em processos industriais e biotecnológicos. Trinta cepas de fungos autóctones foram obtidos em Bornova-Izmir, Turquia. Os extratos frescos dos corpos de frutificação foram submetidos à triagem de atividade de lacase, manganês peroxidase e lignina peroxidase, que são as principais enzimas de degradação de ligninocelulose pelos Basidiomycetes. Foram isolados esporos de Cortinarius sp, Tramnetes versicolor, Pleorotus ostreatus, Abortiporus biennis, Lyophyllum subglobisporium, Ramaria stricta, Ganoderma carnosum, Lactarius delicius ve Lepista desnuda, investigando-se as condições ótimas de cultivo em fermentação submersa para produção de enzimas ligninolíticas com elevado rendimento. Além disso, as cepas fúngicas isoladas foram monitoradas em placas de ágar quanto a capacidade de descoloramento do corante têxtil Remazole Marine Blue.
Key words
Full text:
1
Index:
LILACS
Main subject:
Oxidoreductases
/
Spores, Fungal
/
Basidiomycota
/
Cellulose
/
Coloring Agents
/
Fermentation
/
Fungicides, Industrial
/
Lignin
Type of study:
Diagnostic_studies
/
Screening_studies
Language:
En
Journal:
Braz. j. microbiol
Journal subject:
MICROBIOLOGIA
Year:
2009
Type:
Article