In house colorimetric reverse hybridisation assay for detection of the mutation most frequently associated with resistance to isoniazid in Mycobacterium tuberculosis
Mem. Inst. Oswaldo Cruz
; 104(5): 710-714, Aug. 2009. ilus
Article
in En
| LILACS
| ID: lil-528078
Responsible library:
BR1.1
ABSTRACT
Mutations in the katG gene have been identified and correlated with isoniazid (INH) resistance in Mycobacterium tuberculosis isolates. The mutation AGC→ACC (Ser→Thr) at katG315 has been reported to be the most frequent and is associated with transmission and multidrug resistance. Rapid detection of this mutation could therefore improve the choice of an adequate anti-tuberculosis regimen, the epidemiological monitoring of INH resistance and, possibly, the tracking of transmission of resistant strains. An in house reverse hybridisation assay was designed in our laboratory and evaluated with 180 isolates of M. tuberculosis. It could successfully characterise the katG315 mutation in 100 percent of the samples as compared to DNA sequencing. The test is efficient and is a promising alternative for the rapid identification of INH resistance in regions with a high prevalence of katG315 mutants.
Key words
Full text:
1
Index:
LILACS
Main subject:
Bacterial Proteins
/
Catalase
/
Drug Resistance, Bacterial
/
Isoniazid
/
Mutation
/
Mycobacterium tuberculosis
/
Antitubercular Agents
Type of study:
Diagnostic_studies
/
Risk_factors_studies
Language:
En
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
MEDICINA TROPICAL
/
PARASITOLOGIA
Year:
2009
Type:
Article