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Serological diagnosis of Chagas disease: evaluation and characterisation of a low cost antigen with high sensitivity and specificity
Campos, Yelitza; Briceño, Luis; Reina, Kate; Figarella, Katherine; Pérez, José Luis; Mosca, Walter.
Affiliation
  • Campos, Yelitza; Universidad Central de Venezuela. Facultad de Medicina. Instituto de Biomedicina. Laboratorio de Fisiopatología (Nodo I LANPIP). Caracas. VE
  • Briceño, Luis; Universidad Central de Venezuela. Facultad de Medicina. Instituto de Biomedicina. Laboratorio de Fisiopatología (Nodo I LANPIP). Caracas. VE
  • Reina, Kate; Universidad Central de Venezuela. Facultad de Medicina. Instituto de Biomedicina. Laboratorio de Fisiopatología (Nodo I LANPIP). Caracas. VE
  • Figarella, Katherine; Centro de Biotecnología. Fundación Instituto de Estudios Avanzados. Caracas. VE
  • Pérez, José Luis; Universidad Central de Venezuela. Facultad de Medicina. Instituto de Biomedicina. Laboratorio de Fisiopatología (Nodo I LANPIP). Caracas. VE
  • Mosca, Walter; Universidad Central de Venezuela. Facultad de Medicina. Instituto de Biomedicina. Laboratorio de Fisiopatología (Nodo I LANPIP). Caracas. VE
Mem. Inst. Oswaldo Cruz ; 104(6): 914-917, Sept. 2009. ilus, tab
Article in En | LILACS | ID: lil-529563
Responsible library: BR1.1
ABSTRACT
In spite of evident progress in the serology of Chagas disease, the requirement for new diagnostic antigens persists. We have evaluated different antigens obtained from Trypanosoma cruzi grown in medium rich in nutrients or under nutrient stress, autoclaved or sonicated and fractionated by differential centrifugation. The resulting antigens were evaluated for diagnosis of Chagas disease using ELISA. Immunofluorescence of the parasites demonstrated that nutrient stress induced changes in the distribution and density of antigens recognised by a pool of sera from experimentally infected mice. When evaluated using ELISA, it was evident that most fractions had good sensitivity but poor specificity. Surprisingly, the best specificity and sensitivity was observed with parasites cultured under nutrient stress and autoclaved. Furthermore this antigen had low cross reactivity with sera from other parasitic diseases, Leishmaniasis in particular. Western blot analysis demonstrated that autoclaving seems to non-specifically eliminate cross-reactive antigens. In conclusion, autoclaving epimastigotes of T. cruzi, after nutrient stress, allowed us to obtain an antigen that could be used in the serological diagnosis of Chagas disease.
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Full text: 1 Index: LILACS Main subject: Trypanosoma cruzi / Antibodies, Protozoan / Chagas Disease / Antigens, Protozoan Type of study: Diagnostic_studies / Health_economic_evaluation Limits: Animals / Humans Language: En Journal: Mem. Inst. Oswaldo Cruz Journal subject: MEDICINA TROPICAL / PARASITOLOGIA Year: 2009 Type: Article / Project document
Full text: 1 Index: LILACS Main subject: Trypanosoma cruzi / Antibodies, Protozoan / Chagas Disease / Antigens, Protozoan Type of study: Diagnostic_studies / Health_economic_evaluation Limits: Animals / Humans Language: En Journal: Mem. Inst. Oswaldo Cruz Journal subject: MEDICINA TROPICAL / PARASITOLOGIA Year: 2009 Type: Article / Project document