Response surface methodology for optimization of production of lovastatin by solid state fermentation
Braz. j. microbiol
; 41(1): 164-172, Jan.-Mar. 2010. graf, tab
Article
in En
| LILACS
| ID: lil-531748
Responsible library:
BR32.1
ABSTRACT
Lovastatin, an inhibitor of HMG-CoA reductase, was produced by solid state fermentation (SSF) using a strain of Aspergillus terreus UV 1718. Different solid substrates and various combinations thereof were evaluated for lovastatin production. Wheat bran supported the maximum production (1458 ± 46 µg g-1 DFM) of lovastatin. Response surface methodology (RSM) was applied to optimize the medium constituents. A 2(4) full-factorial central composite design (CCD) was chosen to explain the combined effects of the four medium constituents, viz. moisture content, particle size of the substrate, di-potassium hydrogen phosphate and trace ion solution concentration. Maximum lovastatin production of 2969 µg g-1 DFM was predicted by the quadratic model which was verified experimentally to be 3004 ± 25 µg g-1 DFM. Further RSM optimized medium supplemented with mycological, peptone supported highest yield of 3723.4±49 µg g-1 DFM. Yield of lovastatin increased 2.6 fold as with compared to un-optimized media.
Key words
Full text:
1
Index:
LILACS
Main subject:
Lovastatin
/
Hydroxymethylglutaryl-CoA Reductase Inhibitors
/
Fermentation
Type of study:
Evaluation_studies
/
Prognostic_studies
Language:
En
Journal:
Braz. j. microbiol
Journal subject:
MICROBIOLOGIA
Year:
2010
Type:
Article