Serological diagnosis of paracoccidioidomycosis in HIV-coinfected patients
Mem. Inst. Oswaldo Cruz
; 105(7): 904-907, Nov. 2010. graf, tab
Article
in En
| LILACS
| ID: lil-566181
Responsible library:
BR1.1
ABSTRACT
Paracoccidioidomycosis should be differentiated from other opportunistic diseases in human immunodeficiency virus (HIV)/acquired immunodeficiency syndrome (AIDS) patients who live in Latin America. Laboratory investigation can begin with serological tests, which are rapid and efficient. In the present study, double immunodiffusion (DID), counterimmunoelectrophoresis (CIEP) and an enzyme linked immunosorbent assay (ELISA) tests were assessed for the detection of anti-Paracoccidioides brasiliensis antibodies in 40 patients coinfected with HIV. The results were compared to those obtained for 75 non-HIV-infected patients with endemic paracoccidioidomycosis. Anti-P. brasiliensis antibodies were detected in 65 percent (DID), 79 percent (CIEP) and 95 percent (ELISA) of the patients with HIV/AIDS, significantly lower rates than those detected in cases of endemic paracoccidioidomycosis, which were 89 percent, 99 percent and 100 percent, respectively. The reactive sera of HIV-infected patients also showed lower anti-P. brasiliensis antibody titres than those of non-HIV-infected patients. Despite the lower intensity of the specific humoral response, serological tests are useful for the diagnosis of opportunistic paracoccidioidomycosis in the HIV/AIDS population. We suggest optimization of the laboratory diagnosis by combining the ELISA test with CIEP or DID.
Key words
Full text:
1
Index:
LILACS
Main subject:
Paracoccidioides
/
Paracoccidioidomycosis
/
AIDS-Related Opportunistic Infections
/
Antibodies, Fungal
Type of study:
Diagnostic_studies
Limits:
Adolescent
/
Adult
/
Female
/
Humans
/
Male
Language:
En
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
MEDICINA TROPICAL
/
PARASITOLOGIA
Year:
2010
Type:
Article