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Genotyping of human parvovirus B19 in clinical samples from Brazil and Paraguay using heteroduplex mobility assay, single-stranded conformation polymorphism and nucleotide sequencing
Mendonça, Marcos César Lima de; Ferreira, Ana Maria de Amorim; Santos, Marta Gonçalves Matos dos; Oviedo, Elva Cristina; Bello, Maria Sônia Dal; Siqueira, Marilda Mendonça; Maceira, Juan Manuel Piñeiro; von Hubinger, Maria Genoveva; Couceiro, José Nelson dos Santos Silva.
Affiliation
  • Mendonça, Marcos César Lima de; Centro de Ciências da Saúde. Instituto de Microbiologia Prof. Paulo de Góes.
  • Ferreira, Ana Maria de Amorim; Centro de Ciências da Saúde. Instituto de Microbiologia Prof. Paulo de Góes. Departamento de Virologia.
  • Santos, Marta Gonçalves Matos dos; Centro de Ciências da Saúde. Instituto de Microbiologia Prof. Paulo de Góes. Departamento de Virologia.
  • Oviedo, Elva Cristina; Ministerio de Salud Pública. Laboratorio Central de Salud Pública. Asunción. PY
  • Bello, Maria Sônia Dal; Universidade de Passo Fundo. Faculdade de Medicina. Passo Fundo. BR
  • Siqueira, Marilda Mendonça; Instituto Oswaldo Cruz-Fiocruz. Laboratório de Vírus Respiratórios e Sarampo. Rio de Janeiro. BR
  • Maceira, Juan Manuel Piñeiro; Universidade Federal do Rio de Janeiro. Hospital Universitário Clementino Fraga Filho. Rio de Janeiro. BR
  • von Hubinger, Maria Genoveva; Centro de Ciências da Saúde. Instituto de Microbiologia Prof. Paulo de Góes. Departamento de Virologia.
  • Couceiro, José Nelson dos Santos Silva; Centro de Ciências da Saúde. Instituto de Microbiologia Prof. Paulo de Góes. Departamento de Virologia.
Mem. Inst. Oswaldo Cruz ; 106(4): 502-504, June 2011. ilus
Article in En | LILACS | ID: lil-592195
Responsible library: BR1.1
ABSTRACT
Heteroduplex mobility assay, single-stranded conformation polymorphism and nucleotide sequencing were utilised to genotype human parvovirus B19 samples from Brazil and Paraguay. Ninety-seven serum samples were collected from individuals presenting with abortion or erythema infectiosum, arthropathies, severe anaemia and transient aplastic crisis; two additional skin samples were collected by biopsy. After the procedure, all clinical samples were classified as genotype 1.
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Full text: 1 Index: LILACS Main subject: Parvoviridae Infections / Polymorphism, Single-Stranded Conformational / Heteroduplex Analysis Limits: Female / Humans / Pregnancy Country/Region as subject: America do sul / Brasil / Paraguay Language: En Journal: Mem. Inst. Oswaldo Cruz Journal subject: MEDICINA TROPICAL / PARASITOLOGIA Year: 2011 Type: Article
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Full text: 1 Index: LILACS Main subject: Parvoviridae Infections / Polymorphism, Single-Stranded Conformational / Heteroduplex Analysis Limits: Female / Humans / Pregnancy Country/Region as subject: America do sul / Brasil / Paraguay Language: En Journal: Mem. Inst. Oswaldo Cruz Journal subject: MEDICINA TROPICAL / PARASITOLOGIA Year: 2011 Type: Article