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Taxonomic study and partial characterization of antimicrobial compounds from a moderately halophilic strain of the genus Actinoalloteichus
Boudjelal, Farida; Zitouni, Abdelghani; Mathieu, Florence; Lebrihi, Ahmed; Sabaou, Nasserdine.
  • Boudjelal, Farida; Ecole Normale Supérieure de Kouba. Laboratoire de Recherche sur les Produits Bioactifs et la Valorisation de la Biomasse. DZ
  • Zitouni, Abdelghani; Ecole Normale Supérieure de Kouba. Laboratoire de Recherche sur les Produits Bioactifs et la Valorisation de la Biomasse. DZ
  • Mathieu, Florence; Université de Toulouse. Laboratoire de Génie Chimique. FR
  • Lebrihi, Ahmed; Université de Toulouse. Laboratoire de Génie Chimique. FR
  • Sabaou, Nasserdine; Ecole Normale Supérieure de Kouba. Laboratoire de Recherche sur les Produits Bioactifs et la Valorisation de la Biomasse. DZ
Braz. j. microbiol ; 42(3): 835-845, July-Sept. 2011. ilus, tab
Article in English | LILACS | ID: lil-607512
ABSTRACT
A moderately halophilic actinomycete strain designated AH97 was isolated from a saline Saharan soil, and selected for its antimicrobial activities against bacteria and fungi. The AH97 strain was identified by morphological, chemotaxonomic and phylogenetic analyses to the genus Actinoalloteichus. Analysis of the 16S rDNA sequence of strain AH97 showed a similarity level ranging between 95.8 percent and 98.4 percent within Actinoalloteichus species, with A. hymeniacidonis the most closely related. The comparison of the physiological characteristics of AH97 with those of known species of Actinoalloteichus showed significant differences. Strain AH97 showed an antibacterial and antifungal activity against broad spectrum of microorganisms known to be human and plant pathogens. The bioactive compounds were extracted from the filtrate culture with n-butanol and purified using thin layer chromatography and high pressure liquid chromatography procedures. Two active products were isolated, one hydrophilic fraction (F1) and another hydrophobic (F2). Ultraviolet-visible, infrared, mass and ÕH and 13C nuclear magnetic resonance spectroscopy studies suggested that these molecules were the dioctyl phthalate (F2) and an aminoglycosidic compound (F1).
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: In Vitro Techniques / Biological Factors / Actinobacteria / Biomass / Diethylhexyl Phthalate / Environmental Microbiology Type of study: Prognostic study Language: English Journal: Braz. j. microbiol Journal subject: Microbiology Year: 2011 Type: Article Affiliation country: Algeria / France Institution/Affiliation country: Ecole Normale Supérieure de Kouba/DZ / Université de Toulouse/FR

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Full text: Available Index: LILACS (Americas) Main subject: In Vitro Techniques / Biological Factors / Actinobacteria / Biomass / Diethylhexyl Phthalate / Environmental Microbiology Type of study: Prognostic study Language: English Journal: Braz. j. microbiol Journal subject: Microbiology Year: 2011 Type: Article Affiliation country: Algeria / France Institution/Affiliation country: Ecole Normale Supérieure de Kouba/DZ / Université de Toulouse/FR