Mutaciones asociadas con resistencia a rifampicina o isoniazida en aislamientos clínicos de M. tuberculosis de Sonora, México / DNA mutations associated to rifampicin or isoniazid resistance in M. tuberculosis clinical isolates from Sonora, Mexico
Salud pública Méx
; 54(2): 167-170, marzo-abr. 2012. tab
Article
in Es
| LILACS
| ID: lil-625748
Responsible library:
BR1.1
RESUMEN
OBJETIVO:
Realizar el análisis de regiones específicas de genes asociados con resistencia a isoniazida o rifampicina. MATERIAL YMÉTODOS:
Se estudiaron 22 cepas de M. tuberculosis, aisladas en Sonora, México. Se utilizaron iniciadores para regiones específicas de los genes rpoB, katG e inhA y la región ahpC-oxyR. Los productos de PCR se secuenciaron y analizaron.RESULTADOS:
Se identificaron mutaciones en la región promotora del gen inhA, región ahpC-oxyR, codón 315 del gen katG y codones 451 ó 456 del gen rpoB.CONCLUSIONES:
La identificación de mutaciones no descritas previamente obliga a continuar el análisis genotípico de cepas aisladas en Sonora.ABSTRACT
OBJECTIVE:
To perform the analysis of specific regions of the major genes associated with resistance to isoniazid or rifampin. MATERIALS ANDMETHODS:
Twenty two M. tuberculosis strains, isolated from human samples obtained in Sonora, Mexico. Specific primers for hotspots of the rpoB, katG, inhA genes and the ahpC-oxyR intergenic region were used. The purified PCR products were sequenced.RESULTS:
Mutations in the promoter of inhA, the ahpC-oxyR region, and codon 315 of katG and in 451 or 456 codons of rpoB, were identified.CONCLUSIONS:
Detection of mutations not previously reported requires further genotypic analysis of Mycobacterium tuberculosis isolates in Sonora.Key words
Full text:
1
Index:
LILACS
Main subject:
Rifampin
/
DNA, Bacterial
/
Isoniazid
/
Mutation
/
Mycobacterium tuberculosis
/
Antitubercular Agents
Type of study:
Risk_factors_studies
Limits:
Humans
Country/Region as subject:
Mexico
Language:
Es
Journal:
Salud pública Méx
Journal subject:
SAUDE PUBLICA
Year:
2012
Type:
Article
/
Project document