Cyclic nucleotide phosphodiesterase inhibition increases tyrosine phosphorylation and hyper motility in normal and pathological human spermatozoa
Biocell
; 29(3): 287-293, Aug.-Dec. 2005. ilus, graf, tab
Article
in En
| LILACS
| ID: lil-633235
Responsible library:
AR1.2
ABSTRACT
Our objective was to determine the effect of phosphodiesterase (PDE) inhibition on 1) tyrosine phosphorylation of human spermatozoa at the tail level; and 2) sperm motion parameters and hyperactivated motility. The study was conducted with normozoospermic and asthenozoospermic samples incubated under in vitro capacitating conditions. The main outcome measures were computer-assisted sperm motion analysis and fluorescent immunodetection of phosphotyrosine-containing proteins. Pentoxifylline (PTX) was used as PDE inhibitor because of its wide use in the clinic. PTX-treatment significantly increased sperm velocity, hyperactivated motility and tyrosine-phosphorylation, both in normo and asthenozoospermic samples. Tyrosine-phosphorylation of tail proteins was highly conspicuous in both types of samples, showing no differential pattern after PTX-treatment. Normozoospermic samples treated with pentoxifylline showed an increase in the number of spermatozoa displaying hyperactivated movement and tyrosine-phosphorylation at the tail level. Preliminary data on asthenozoospermic samples exhibiting altered motion characteristics and defective phosphorylation of sperm-tail proteins showed that both defects can be concomitantly overcome by pentoxifylline treatment. Tyrosine-phosphorylation of sperm-tail proteins is underlying the enhancement of hyperactivated motility resulting from PDE inhibition by pentoxifylline.
Key words
Full text:
1
Index:
LILACS
Main subject:
Pentoxifylline
/
Phosphodiesterase Inhibitors
/
Sperm Motility
/
Spermatozoa
/
Tyrosine
Limits:
Humans
/
Male
Language:
En
Journal:
Biocell
Journal subject:
Clulas
Year:
2005
Type:
Article