A comparison of silver staining protocols for detecting DNA in polyester-backed polyacrylamide gel
Braz. j. microbiol
; 43(2): 649-652, Apr.-June 2012. ilus, tab
Article
in En
| LILACS
| ID: lil-644482
Responsible library:
BR32.1
ABSTRACT
Eight silver-staining protocols were applied to detect DNA in polyester-backed gels to select the optimal. Results showed important differences in staining quality and that four methods were well-suited for TGGE gels due to high sensitivity and low background, including the Bassam et al. methods, the manufacturer method and our improved method.
Key words
Full text:
1
Index:
LILACS
Main subject:
Polyesters
/
Silver
/
Staining and Labeling
/
In Vitro Techniques
/
DNA
/
Soil Alkalinity
/
Coloring Agents
/
Gels
Type of study:
Guideline
Language:
En
Journal:
Braz. j. microbiol
Journal subject:
MICROBIOLOGIA
Year:
2012
Type:
Article
/
Project document