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A comparison of silver staining protocols for detecting DNA in polyester-backed polyacrylamide gel
Qiu, Shanlian; Chen, Jichen; Lin, Si; Lin, Xinjian.
Affiliation
  • Qiu, Shanlian; Soil and Fertilizer Institute. Fujian Academy of Agricultural Sciences. Fuzhou. CN
  • Chen, Jichen; Soil and Fertilizer Institute. Fujian Academy of Agricultural Sciences. Fuzhou. CN
  • Lin, Si; Fuzhou University. College of biological science and technology. Fuzhou. CN
  • Lin, Xinjian; Soil and Fertilizer Institute. Fujian Academy of Agricultural Sciences. Fuzhou. CN
Braz. j. microbiol ; 43(2): 649-652, Apr.-June 2012. ilus, tab
Article in En | LILACS | ID: lil-644482
Responsible library: BR32.1
ABSTRACT
Eight silver-staining protocols were applied to detect DNA in polyester-backed gels to select the optimal. Results showed important differences in staining quality and that four methods were well-suited for TGGE gels due to high sensitivity and low background, including the Bassam et al. methods, the manufacturer method and our improved method.
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Full text: 1 Index: LILACS Main subject: Polyesters / Silver / Staining and Labeling / In Vitro Techniques / DNA / Soil Alkalinity / Coloring Agents / Gels Type of study: Guideline Language: En Journal: Braz. j. microbiol Journal subject: MICROBIOLOGIA Year: 2012 Type: Article / Project document
Fulltext
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Print
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Full text: 1 Index: LILACS Main subject: Polyesters / Silver / Staining and Labeling / In Vitro Techniques / DNA / Soil Alkalinity / Coloring Agents / Gels Type of study: Guideline Language: En Journal: Braz. j. microbiol Journal subject: MICROBIOLOGIA Year: 2012 Type: Article / Project document