Rapid in vitro plant regeneration from leaf explants of Launaea sarmentosa (Willd.) Sch. Bip. ex Kuntze
Biol. Res
; 45(2): 131-136, 2012. ilus, tab
Article
in En
| LILACS
| ID: lil-648571
Responsible library:
CL1.1
ABSTRACT
An efficient protocol for organogenesis through leaves has been established for Launaea sarmentosa (Willd.) Sch. Bip. ex Kuntze, a highly valuable medicinal plant. The leaf explants produced microshoots on MS basal medium when fortified with cytokinins and auxins. A combination of 6-benzylaminopurine (BAP) at 0.5mg/l and naphthaleneacetic acid (NAA) at 0.2mg/l resulted in the induction of high frequency microshoots in 30 days. The microshoots were successfully subcultured for shoot elongation and eventually for rooting on MS medium supplemented with indole-3-butyric acid (IBA) at 0.5mg/l. The regenerated plantlets were hardened under greenhouse conditions and transferred to garden, resulting in a 90% survival rate.
Key words
Full text:
1
Index:
LILACS
Main subject:
Plant Growth Regulators
/
Regeneration
/
Plant Shoots
/
Plant Leaves
/
Asteraceae
/
Organogenesis, Plant
Language:
En
Journal:
Biol. Res
Journal subject:
BIOLOGIA
Year:
2012
Type:
Article