Simultaneous environmental manipulations in semi-perfusion cultures of CHO cells producing rh-tPA
Electron. j. biotechnol
; Electron. j. biotechnol;15(6): 2-2, Nov. 2012. ilus, tab
Article
in En
| LILACS
| ID: lil-662200
Responsible library:
CL1.1
ABSTRACT
We evaluated the combined effect of decreasing the temperature to a mild hypothermia range (34 and 31ºC) and switching to a slowly metabolizable carbon source (glucose substituted by galactose) on the growth and production of a recombinant human tissue plasminogen activator (rh-tPA) by Chinese hamster ovary cells in batch and semi-perfusion cultures. In batch cultures using glucose as a carbon source, decreasing the temperature caused a reduction in cell growth and an increase in specific productivity of rh-tPA of 32 percent at 34ºC and 55 percent at 31ºC, compared to cultures at 37ºC. Similar behaviour was observed in cultures at 34ºC using galactose as a carbon source. Nonetheless, at 31ºC, the specific productivity of rh-tPA strongly decreased (about 58 percent) compared to the culture at 37ºC. In semi-perfusion culture, the highest rh-tPA specific productivity was obtained at 34ºC. Similarly, whether a decrease in the temperature is accompanied of the replacement of glucose by galactose, the rh-tPA specific productivity improved about 112 percent over that obtained in semi-perfusion culture carried out at 37ºC with glucose as the carbon source. A semi-perfusion culture strategy was implemented based on the combined effect of the chosen carbon source and low temperatures, which was a useful approach for enhance the specific productivity of the recombinant protein.
Key words
Full text:
1
Index:
LILACS
Main subject:
CHO Cells
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Tissue Plasminogen Activator
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Cold Temperature
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Glutamic Acid
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Galactose
Language:
En
Journal:
EJB
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Electron. j. biotechnol
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Electronic journal of biotechnology
Journal subject:
BIOTECNOLOGIA
Year:
2012
Type:
Article
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Project document