A simple and effective pressure culture system modified from a transwell cell culture system
Biol. Res
;
46(1): 47-52, 2013. ilus
Article
in English
| LILACS
| ID: lil-676820
ABSTRACT
Mechanical pressure plays an important role in many physiological and pathological processes. Mimicking the mechanical pressure present in vitro is necessary for related research, but usually requires expensive and complicated equipment. In this study we created a simple pressure culture system based on the transwell culture system. By cutting off the top rim of the transwell insert, the cells were compressed between the insert membrane and the well floor. The new pressure culture system was proven effective in that it induced cell morphological change, integrin β1 upregulation, actin polymerization and growth change in rat retinal ganglion cells, human nasopharyngeal carcinoma cells and mice embryonic fibroblasts. Though the pressure value is immeasurable and inhomogeneous, the easily available culture system still provides a choice for the laboratories that do not have access to the better, but much more expensive pressure culture equipment.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Cell Culture Techniques
/
Cell Proliferation
Limits:
Animals
/
Humans
Language:
English
Journal:
Biol. Res
Journal subject:
Biology
Year:
2013
Type:
Article
/
Project document
Affiliation country:
China
Institution/Affiliation country:
Guangdong Medical College/CN
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