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SEROLOGICAL SURVEY OF Ehrlichia SPECIES IN DOGS, HORSES AND HUMANS: ZOONOTIC SCENERY IN A RURAL SETTLEMENT FROM SOUTHERN BRAZIL / Investigação sorológica de espécies de Ehrlichia em cães, equinos e humanos de um assentamento rural do sul do Brasil
Revista do Instituto de Medicina Tropical de Sao Paulo; Vieira, Rafael Felipe da Costa; Vieira, Thallitha Samih Wischral Jayme; Nascimento, Denise do Amaral Gomes; Martins, Thiago F.; Krawczak, Felipe S.; Labruna, Marcelo B.; Chandrashekar, Ramaswamy; Marcondes, Mary; Biondo, Alexander Welker; Vidotto, Odilon.
Affiliation
  • Vieira, Rafael Felipe da Costa; s.af
  • Vieira, Thallitha Samih Wischral Jayme; s.af
  • Nascimento, Denise do Amaral Gomes; s.af
  • Martins, Thiago F.; s.af
  • Krawczak, Felipe S.; s.af
  • Labruna, Marcelo B.; s.af
  • Chandrashekar, Ramaswamy; s.af
  • Marcondes, Mary; s.af
  • Biondo, Alexander Welker; s.af
  • Vidotto, Odilon; s.af
Rev. Inst. Med. Trop. Säo Paulo ; Rev. Inst. Med. Trop. Säo Paulo;55(5): 335-340, Sep-Oct/2013. tab
Article in En | LILACS | ID: lil-685550
Responsible library: BR1.1
ABSTRACT
SUMMARY The aims of this study were to determine the seroprevalence of Ehrlichia spp. and risk factors for exposure in a restricted population of dogs, horses, and humans highly exposed to tick bites in a Brazilian rural settlement using a commercial ELISA rapid test and two indirect immunofluorescent assays (IFA) with E. canis and E. chaffeensis crude antigens. Serum samples from 132 dogs, 16 horses and 100 humans were used. Fifty-six out of 132 (42.4%) dogs were seropositive for E. canis. Dogs > one year were more likely to be seropositive for E. canis than dogs ≤ one year (p = 0.0051). Ten/16 (62.5%) and 8/16 (50%) horses were seropositive by the commercial ELISA and IFA, respectively. Five out of 100 (5%) humans were seropositive for E. canis and E. chaffeensis. Rhipicephalus sanguineus (n = 291, 97.98%) on dogs and Amblyomma cajennense (n = 25, 96.15%) on horses were the most common ticks found. In conclusion, anti-Ehrlichia spp. antibodies were found in horses; however, the lack of a molecular characterization precludes any conclusion regarding the agent involved. Additionally, the higher seroprevalence of E. canis in dogs and the evidence of anti-Ehrlichia spp. antibodies in humans suggest that human cases of ehrlichiosis in Brazil might be caused by E. canis, or other closely related species. .
RESUMO
RESUMO Objetivou-se determinar a soroprevalência de Ehrlichia spp. e os fatores de risco associados a exposição em uma população restrita de cães, cavalos e humanos altamente expostos a picadas de carrapatos em um assentamento rural brasileiro utilizando um teste comercial de ELISA rápido e dois testes de imunofluorescência indireta (IFI) com antígenos brutos de E. canis e E. chaffeensis. Amostras de soro de 132 cães, 16 cavalos e 100 humanos foram utilizadas. Cinquenta e seis/132 (42,4%) cães foram soropositivos para E. canis. Cães > um ano apresentaram mais chance de serem soropositivos para E. canis do que cães ≤ um ano (p = 0,0051). Dez/16 (62,5%) e 8/16 (50%) cavalos foram soropositivos pelo ELISA comercial e IFI, respectivamente. Cinco/100 (5%) humanos foram soropositivos para E. canis e E. chaffeensis. Rhipicephalus sanguineus (n = 291, 97,98%) nos cães e A. cajennense (n = 25, 96,15%) nos cavalos foram os carrapatos mais encontrados. Concluindo, anticorpos anti-Ehrlichia spp. foram encontrados em cavalos; entretanto, a ausência de uma caracterização molecular impede qualquer conclusão sobre agente envolvido. Além disso, a alta soroprevalência de E. canis em cães e a evidência de anticorpos anti-Ehrlichia sp. em humanos, sugere que os casos de erliquiose humana no Brasil possam ser causados por E. canis ou outra espécie intimamente relacionada. .
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Full text: 1 Index: LILACS Main subject: Dogs / Ehrlichia / Horses / Antibodies, Bacterial Type of study: Etiology_studies / Risk_factors_studies Limits: Animals / Female / Humans / Male Country/Region as subject: America do sul / Brasil Language: En Journal: Rev. Inst. Med. Trop. Säo Paulo Journal subject: MEDICINA TROPICAL Year: 2013 Type: Article

Full text: 1 Index: LILACS Main subject: Dogs / Ehrlichia / Horses / Antibodies, Bacterial Type of study: Etiology_studies / Risk_factors_studies Limits: Animals / Female / Humans / Male Country/Region as subject: America do sul / Brasil Language: En Journal: Rev. Inst. Med. Trop. Säo Paulo Journal subject: MEDICINA TROPICAL Year: 2013 Type: Article