Comparison of the direct fluorescence assay and real-time polymerase chain reaction for the detection of influenza virus A and B in immunocompromised patients
Clinics
;
68(9): 1206-1209, set. 2013. tab, graf
Article
in English
| LILACS
| ID: lil-687769
ABSTRACT
OBJECTIVE:
This study evaluated the diagnostic performance of two methods for the detection of influenza virus in immunocompromised transplant patients.METHODS:
A total of 475 respiratory samples, 236 from patients in a hematopoietic stem cell transplantation program and 239 from kidney transplant patients, were analyzed by a direct fluorescence assay and the Centers for Disease Control real-time polymerase chain reaction protocol for influenza A and B detection.RESULTS:
Influenza detection using either method was 7.6% in the hematopoietic stem cell transplant group and 30.5% in the kidney transplant patient group. Influenza detection by real-time polymerase chain reaction yielded a higher positive rate compared with fluorescence than that reported by other studies, and this difference was more pronounced for influenza A. The fluorescence assay sensitivity, specificity, positive and negative predictive values, and kappa coefficient were 17.6%, 100%, 1, 0.83, and 0.256, respectively, and lower detection rates occurred in the kidney transplant patients.CONCLUSIONS:
The real-time polymerase chain reaction performance and the associated turnaround time for a large number of samples support the choice of this method for use in different routine diagnostic settings and influenza surveillance in high-risk patients. .
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Influenza A virus
/
Influenza B virus
/
Immunocompromised Host
/
Fluorescent Antibody Technique, Direct
/
Influenza, Human
/
Real-Time Polymerase Chain Reaction
Type of study:
Diagnostic study
/
Etiology study
/
Evaluation studies
/
Practice guideline
/
Observational study
/
Prognostic study
/
Risk factors
Limits:
Adult
/
Humans
Language:
English
Journal:
Clinics
Journal subject:
Medicine
Year:
2013
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Federal University of Sao Paulo/BR
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