A multiparametric assay to compare the cytotoxicity of different storage media for avulsed teeth
Braz. j. oral sci
; 12(2): 90-94, Apr.-June 2013. ilus
Article
in En
| LILACS, BBO
| ID: lil-694424
Responsible library:
BR218.1
ABSTRACT
Aim:
To evaluate the cytotoxicity of several storage media (coconut water, whole milk, isotonicsolution, Hank´s Balanced Salt Solution and tap water) using a multiparametric cytotoxicity analysisemploying 3T3 cells.Methods:
Plates containing confluent 3T3 fibroblasts were exposed to thevarious media for 24h, at 37°C with 5% CO2, and cell viability was evaluated by a multiparametricassay assessing sequentially, on the same cells, mitochondrial activity (XTT), membrane integrity(neutral red test), and total cell density (crystal violet dye exclusion test). Results from each testwere compared by one-way analysis of variance (ANOVA).Results:
Statistical analysis showedthat whole milk and HBSS were the most effective media in maintaining cell viability at all testedtimes (p<0.05). Isotonic and tap water showed the highest cytotoxicity effects.Conclusions:
Thisstudy shows that whole milk and HBSS are more efficient in maintaining the viability of 3T3fibroblasts as demonstrated by three different cell viability tests.
Full text:
1
Index:
LILACS
Main subject:
Tooth Avulsion
/
Cell Survival
/
Fibroblasts
Language:
En
Journal:
Braz. j. oral sci
Journal subject:
ODONTOLOGIA
Year:
2013
Type:
Article