A simple, rapid and economic method for detecting multidrug-resistant tuberculosis
Braz. j. infect. dis
; 17(6): 667-671, Nov.-Dec. 2013. ilus, tab
Article
in En
| LILACS
| ID: lil-696968
Responsible library:
BR1.1
ABSTRACT
OBJECTIVE:
To evaluate multiplex allele specific polymerase chain reaction as a rapid molecular tool for detecting multidrug-resistant tuberculosis.METHODS:
Based on drug susceptibility testing, 103 isolates were multidrug-resistant tuberculosis and 45 isolates were sensitive to isonicotinylhydrazine and rifampin. Primers were designed to target five mutations hotspots that confer resistance to the first-line drugs isoniazid and rifampin, and multiplex allele specific polymerase chain reaction was performed. Whole-genome sequencing confirmed drug resistance mutations identified by multiplex allele specific polymerase chain reaction.RESULTS:
DNA sequencing revealed that 68.9% of multidrug-resistant strains have point mutations at codon 315 of the katG gene, 19.8% within the mabA-inhA promoter, and 98.0% at three hotspots within rpoB. Multiplex allele specific polymerase chain reaction detected each of these five mutations, yielding 82.3% sensitivity and 100% specificity for isoniazid resistance, and 97.9% sensitivity and 100% specificity for rifampin resistance as compared to drug susceptibility testing.CONCLUSIONS:
The results show that multiplex allele specific polymerase chain reaction is an inexpensive and practical method for rapid detection of multidrug-resistant tuberculosis in developing countries.Key words
Full text:
1
Index:
LILACS
Main subject:
Tuberculosis, Multidrug-Resistant
/
Multiplex Polymerase Chain Reaction
/
Mycobacterium tuberculosis
/
Antitubercular Agents
Type of study:
Diagnostic_studies
/
Health_economic_evaluation
/
Prognostic_studies
Limits:
Humans
Language:
En
Journal:
Braz. j. infect. dis
Journal subject:
DOENCAS TRANSMISSIVEIS
Year:
2013
Type:
Article