PCR and ELISA (VIDAS ECO O157®) Escherichia coli O157:H7 identification in Minas Frescal cheese commercialized in Goiânia, GO
Braz. j. microbiol
;
45(1): 7-10, 2014. ilus
Article
in English
| LILACS
| ID: lil-709473
ABSTRACT
Escherichia coli O157H7 has been incriminated in food poisoning outbreaks and sporadic cases of hemorrhagic colitis and hemolytic uremic syndrome in many countries. Considering the high susceptibility of Minas Frescal cheese to contamination by E. coli O157H7, the aim of this study was to determine the occurrence of this pathogen through PCR (Polymerase Chain Reaction) and ELISA (VIDAS ECO O157®, bioMérieux, Lyon, France) test. Thirty cheese samples manufactured by artisan farmhouse producers were collected from open-air markets in Goiânia and thirty from industries under Federal Inspection located in Goiás State which trade their products in supermarkets in Goiânia. E. coli O157H7 was detected in 6.67% samples collected in open air markets using ELISA, and 23,33% with PCR. The pathogen was not detected in samples from industries under Federal Inspection.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Cheese
/
Polymerase Chain Reaction
Type of study:
Diagnostic study
/
Prevalence study
/
Risk factors
Limits:
Humans
Language:
English
Journal:
Braz. j. microbiol
Journal subject:
Microbiology
Year:
2014
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Embrapa Arroz e Feijão/BR
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