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Exogenous ATP administration prevents ischemia/reperfusion-induced oxidative stress and tissue injury by modulation of hypoxanthine metabolic pathway in rat ovary / Administração exógena de ATP impede isquemia/induzidas por reperfusão de estresse oxidativo de tecidos e lesões pela modulação da hipoxantina via metabólica em ovários de ratos
Kumbasar, Serkan; Cetin, Nihal; Yapca, Omer Erkan; Sener, Ebru; Isaoglu, Unal; Yilmaz, ehmet; Salman, Suleyman; Aksoy, Ayse Nur; Gul, Mehmet Ali; Suleyman, Halis.
Affiliation
  • Kumbasar, Serkan; Sakarya University. Faculty of Medicine. Department of Obstetrics and Gynecology. Sakarya. TR
  • Cetin, Nihal; Sakarya University. Faculty of Medicine. Department of Obstetrics and Gynecology. Sakarya. TR
  • Yapca, Omer Erkan; Sakarya University. Faculty of Medicine. Department of Obstetrics and Gynecology. Sakarya. TR
  • Sener, Ebru; Sakarya University. Faculty of Medicine. Department of Obstetrics and Gynecology. Sakarya. TR
  • Isaoglu, Unal; Sakarya University. Faculty of Medicine. Department of Obstetrics and Gynecology. Sakarya. TR
  • Yilmaz, ehmet; Sakarya University. Faculty of Medicine. Department of Obstetrics and Gynecology. Sakarya. TR
  • Salman, Suleyman; Sakarya University. Faculty of Medicine. Department of Obstetrics and Gynecology. Sakarya. TR
  • Aksoy, Ayse Nur; Sakarya University. Faculty of Medicine. Department of Obstetrics and Gynecology. Sakarya. TR
  • Gul, Mehmet Ali; Sakarya University. Faculty of Medicine. Department of Obstetrics and Gynecology. Sakarya. TR
  • Suleyman, Halis; Sakarya University. Faculty of Medicine. Department of Obstetrics and Gynecology. Sakarya. TR
Ciênc. rural ; Ciênc. rural (Online);44(7): 1257-1263, 07/2014. tab, graf
Article in En | LILACS | ID: lil-718170
Responsible library: BR1.1
ABSTRACT
In this study, xanthine oxidase (XO), malondialdehyde (MDA), myeloperoxidase (MPO) and glutathione (GSH) levels in the ovarian tissues of rats during the development of ischemia and postischemia-induced reperfusion were investigated, and the effect of ATP on ischemia-reperfusion (I/R) damage was biochemically and histopathologically examined. The results of the biochemical analyses demonstrated that ATP significantly reduced the level of XO and MDA and increased the amount of GSH in both ischemia and I/R-applied ovarian tissue at the doses administered. Furthermore, ATP significantly suppressed the increase in MPO activity that occurred following the application of post ischemia reperfusion in the ovarian tissue. The biochemical results obtained in the present study coincide with the histological findings. The severity of the pathological findings, such as dilatation, congestion, haemorrhage, oedema and polymorphonuclear nuclear leukocytes (PMNLs), increased in parallel with the increase observed in the products of XO metabolism. In conclusion, exogenously applied ATP prevented I/R damage by reducing the formation of XO in ischemic ovarian tissue.
RESUMO
Neste estudo, a xantina oxidase (XO), o malondialdeído (MDA), mieloperoxidase ( MPO ) e glutationa ( GSH) nos tecidos do ovário de ratos, durante o desenvolvimento de isquemia e reperfusão induzida por pós-isquemia foi investigada, e o efeito de ATP em isquemia e reperfusão (I/R). O dano foi verificado por provas bioquímicas e por histopatologia. Os resultados das análises bioquímicas mostraram que o ATP reduziu significativamente o nível de XO e MDA e aumentou a quantidade de GSH em ambas as isquemia e no tecido do ovário de I / R - aplicado nas doses administradas. Além disso, o ATP suprimiu significativamente o aumento na atividade de MPO que ocorreu na sequência da aplicação de pós-isquemia reperfusão no tecido ovariano. Os resultados bioquímicos obtidos no presente estudo coincidem com os achados histológicos. A gravidade dos achados patológicos, como a dilatação, congestão, hemorragia, edema e polimorfonucleares leucócitos nucleares (PMNLs), aumentou em paralelo com o aumento observado nos produtos do metabolismo XO. Em conclusão, aplicando exogenamente ATP impedido de I/R, houve danos pela redução da formação de tecido de ovário de XO na isquemia.
Key words

Full text: 1 Index: LILACS Language: En Journal: Ciênc. rural / Ciênc. rural (Online) Journal subject: CIENCIA / SAUDE AMBIENTAL Year: 2014 Type: Article

Full text: 1 Index: LILACS Language: En Journal: Ciênc. rural / Ciênc. rural (Online) Journal subject: CIENCIA / SAUDE AMBIENTAL Year: 2014 Type: Article