L-FABP and I-FABP expression in newborn rats changes inversely in the model of necrotizing enterocolitis
Acta cir. bras
;
29(supl.2): 43-49, 2014. tab, graf
Article
in English
| LILACS
| ID: lil-721381
ABSTRACT
PURPOSE:
To determine the expression of hepatic L-FABP and intestinal I-FABP in an experimental model of necrotizing enterocolitis (NEC) in neonatal rats.METHODS:
Newborn Sprague-Dawley rats were divided into four groups Control (C1) - exclusive breastfeeding at the first and sixth procedures (C6), NEC1 - fed formula milk and submitted to hypoxia and hypothermia at the first and sixth procedures (NEC6). The newborn pups were fed twice a day for three days, for a total of six procedures. Samples were collected for morphometric evaluation (body weight, liver weight, liver weight/body weight ratio, intestinal weight and intestinal/body weight ratio) and for immunohistochemical and Western blotting analysis. The values obtained were analyzed statistically, with the level of significance set at p<0.05.RESULTS:
Morphometric measurements showed reduction of body and liver weights in the NEC group (p<0.05). Both immunohistochemistry and western blotting revealed that L-FABP expression in the liver was decreased and I-FABP expression in the ileum was increased in the NEC group (p<0.05).CONCLUSION:
L-FABP and I-FABP expression changed inversely in the rat NEC model. These findings can contribute to a better diagnosis of NEC in human newborns. .
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Enterocolitis, Necrotizing
/
Fatty Acid-Binding Proteins
/
Ileum
/
Liver
Type of study:
Prognostic study
Limits:
Animals
Language:
English
Journal:
Acta cir. bras
Journal subject:
General Surgery
/
Procedimentos Cir£rgicos Operat¢rios
Year:
2014
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
University of Sao Paulo/BR
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