Bioinformatics based structural characterization of glucose dehydrogenase (gdh) gene and growth promoting activity of Leclercia sp. QAU-66
Braz. j. microbiol
;
45(2): 603-611, Apr.-June 2014. ilus, tab
Article
in English
| LILACS
| ID: lil-723124
ABSTRACT
Glucose dehydrogenase (GDH; EC 1.1. 5.2) is the member of quinoproteins group that use the redox cofactor pyrroloquinoline quinoine, calcium ions and glucose as substrate for its activity. In present study, Leclercia sp. QAU-66, isolated from rhizosphere of Vigna mungo, was characterized for phosphate solubilization and the role of GDH in plant growth promotion of Phaseolus vulgaris. The strain QAU-66 had ability to solubilize phosphorus and significantly (p < 0.05) promoted the shoot and root lengths of Phaseolus vulgaris. The structural determination of GDH protein was carried out using bioinformatics tools like Pfam, InterProScan, I-TASSER and COFACTOR. These tools predicted the structural based functional homology of pyrroloquinoline quinone domains in GDH. GDH of Leclercia sp. QAU-66 is one of the main factor that involved in plant growth promotion and provides a solid background for further research in plant growth promoting activities.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Phaseolus
/
Glucose 1-Dehydrogenase
/
Enterobacteriaceae
/
Nerve Growth Factors
Type of study:
Prognostic study
Language:
English
Journal:
Braz. j. microbiol
Journal subject:
Microbiology
Year:
2014
Type:
Article
Affiliation country:
Pakistan
Institution/Affiliation country:
Quaid-i-Azam University/PK
Similar
MEDLINE
...
LILACS
LIS