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Small activating RNA induces myogenic differentiation of rat adipose-derived stem cells by upregulating MyoD
Wang, Chenghe; Chen, Zhong; Wu, Jia; Zhang, Yan; Hu, Jia; Ge, Qiangqiang; Wang, Tao; Yang, Weimin; Xu, Hua; Liu, Jihong; Ye, Zhangqun.
Affiliation
  • Wang, Chenghe; Huazhong University of Science and Technology. Tongji Medical College. Department of Urology, Tongji Hospital. Hubei. CN
  • Chen, Zhong; Huazhong University of Science and Technology. Tongji Medical College. Department of Urology, Tongji Hospital. Hubei. CN
  • Wu, Jia; Huazhong University of Science and Technology. Tongji Medical College. Department of Urology, Tongji Hospital. Hubei. CN
  • Zhang, Yan; Huazhong University of Science and Technology. Tongji Medical College. Department of Urology, Tongji Hospital. Hubei. CN
  • Hu, Jia; Huazhong University of Science and Technology. Tongji Medical College. Department of Urology, Tongji Hospital. Hubei. CN
  • Ge, Qiangqiang; Huazhong University of Science and Technology. Tongji Medical College. Department of Urology, Tongji Hospital. Hubei. CN
  • Wang, Tao; Huazhong University of Science and Technology. Tongji Medical College. Department of Urology, Tongji Hospital. Hubei. CN
  • Yang, Weimin; Huazhong University of Science and Technology. Tongji Medical College. Department of Urology, Tongji Hospital. Hubei. CN
  • Xu, Hua; Huazhong University of Science and Technology. Tongji Medical College. Department of Urology, Tongji Hospital. Hubei. CN
  • Liu, Jihong; Huazhong University of Science and Technology. Tongji Medical College. Department of Urology, Tongji Hospital. Hubei. CN
  • Ye, Zhangqun; Huazhong University of Science and Technology. Tongji Medical College. Department of Urology, Tongji Hospital. Hubei. CN
Int. braz. j. urol ; 41(4): 764-772, July-Aug. 2015. graf
Article in En | LILACS | ID: lil-763064
Responsible library: BR1.1
ABSTRACT
ABSTRACT

Purpose:

RNA activation (RNAa) is a mechanism of gene activation triggered by promoter-targeted small double stranded RNAs (dsRNAs), also known as small activating RNAs (saRNAs). Myogenic regulatory factor MyoD is regarded as the master activator of myogenic differentiation cascade by binding to enhancer of muscle specific genes. Stress urinary incontinence (SUI) is a condition primarily resulted from urethral sphincter deficiency. It is thus expected that by promoting differentiation of adipose-derived stem cells (ADSCs) into myoblasts by activating MyoD gene through RNAa may offer benefits to SUI.Materials and

Methods:

Rats ADSCs were isolated, proliferated in vitro, and identified by flow cytometry. Purified ADSCs were then transfected with a MyoD saRNA or control transfected. Real-time polymerase chain reaction (RT-PCR) and western blotting were used to detect MyoD mRNA and protein expression, respectively. Immunocytochemical staining was applied to determine the expression of desmin protein in transfected cells. Cell viability was measured by using CellTiter 96® AQueous One Solution Cell Proliferation Assay kit.

Results:

Transfection of a MyoD saRNA (dsMyoD) into ADSCs significantly induced the expression of MyoD at both the mRNA and protein levels, and inhibited cell proliferation. Desmin protein expression was detected in dsMyoD treated ADSCs 2 weeks later.

Conclusion:

Our findings show that RNAa mediated overexpression of MyoD can promote transdifferentiation of ADSCs into myoblasts and may help treat stress urinary incontinence (SUI)–a condition primarily resulted from urethral sphincter deficiency.
Subject(s)
Key words

Full text: 1 Index: LILACS Main subject: Stem Cells / RNA, Double-Stranded / Cell Differentiation / Adipose Tissue / MyoD Protein / Myoblasts / Desmin Type of study: Prognostic_studies Limits: Animals Language: En Journal: Int. braz. j. urol Journal subject: UROLOGIA Year: 2015 Type: Article / Project document

Full text: 1 Index: LILACS Main subject: Stem Cells / RNA, Double-Stranded / Cell Differentiation / Adipose Tissue / MyoD Protein / Myoblasts / Desmin Type of study: Prognostic_studies Limits: Animals Language: En Journal: Int. braz. j. urol Journal subject: UROLOGIA Year: 2015 Type: Article / Project document