Static compression regulates OPG expression in periodontal ligament cells via the CAMK II pathway
J. appl. oral sci
; 23(6): 549-554, Nov.-Dec. 2015. graf
Article
in En
| LILACS, BBO
| ID: lil-769817
Responsible library:
BR1.1
ABSTRACT
ABSTRACT Objective This study aimed to investigate the potential role of CAMK II pathway in the compression-regulated OPG expression in periodontal ligament cells (PDLCs). Material and Methods The PDL tissue model was developed by 3-D culturing human PDLCs in a thin sheet of poly lactic-co-glycolic acid (PLGA) scaffolds, which was subjected to static compression of 25 g/cm2 for 3, 6 and 12 h, with or without treatment of KN-93. After that, the expression of OPG, RANKL and NFATC2 was investigated through real-time PCR and western blot analysis. Results After static compression, the NFATC2 and RANKL expression was significantly up-regulated, while partially suppressed by KN-93 for 6 and 12 h respectively. The OPG expression was significantly down-regulated by compression in 3 h, started to elevate in 6 h, and significantly up-regulated in 12 h. The up-regulation after 12 h was significantly suppressed by KN-93. Conclusions Long-term static compression increases OPG expression in PDLCs, at least partially, via the CAMK II pathway.
Key words
Full text:
1
Index:
LILACS
Main subject:
Osteogenesis
/
Periodontal Ligament
/
Osteoprotegerin
Type of study:
Clinical_trials
/
Prognostic_studies
Limits:
Humans
Language:
En
Journal:
J. appl. oral sci
Journal subject:
ODONTOLOGIA
Year:
2015
Type:
Article