Effect of ozone on colon anastomoses in rat peritonitis model
Acta cir. bras
; Acta cir. bras;31(2): 111-118, Feb. 2016. graf
Article
in En
| LILACS
| ID: lil-775561
Responsible library:
BR1.1
ABSTRACT
PURPOSE:
To investigate the effects of medical ozone theraphy on the colon anastomosis of peritonitis model in rats.METHODS:
Eighteen rats were randomly assigned into three equal groups; control, cecal punctuation and colon anastomosis and ozone theraphy. Sepsis was performed with a cecal punctuation in groups 2 and 3. The medical ozone theraphy was administered intraperitonealy for three weeks in group 3 while the other rats received saline injection. At the twenty second day serum were obtained for TNF-α and IL-1β, the colonic burst pressures were measured and colonic tissue samples were obtained for MDA and MPO levels. Histolopatological examination was evaluated with H&E stain, and Ki-67, IL-1β and the VEGF immunostaining densities were also compared.RESULTS:
Intraperitoneal ozone administration reversed TNF-α, IL-1β, MDA and MPO levels and the colonic burst pressures. There was also a significant difference at immunostaining densities of histopathological examination.CONCLUSION:
Medical ozone therapy may contribute to tissue healing by affecting the proliferation and the vascularization thus has benefits on colonic anastomosis at peritonitis in rats.Key words
Full text:
1
Index:
LILACS
Main subject:
Ozone
/
Peritonitis
/
Wound Healing
/
Colon
Type of study:
Prognostic_studies
Limits:
Animals
Language:
En
Journal:
Acta cir. bras
Journal subject:
Cirurgia Geral
/
Procedimentos Cir£rgicos Operat¢rios
Year:
2016
Type:
Article