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Allopurinol preconditioning attenuates renal ischemia/reperfusion injury by inhibiting HMGB1 expression in a rat model
Zhou, Jiang-qiao; Qiu, Tao; Zhang, Lu; Chen, Zhong-bao; Wang, Zhi-shun; Ma, Xiao-xiong; Li, Dongyu.
Affiliation
  • Zhou, Jiang-qiao; Wuhan University. Renmin Hospital. Department of Organ Transplantation. Wuhan. CN
  • Qiu, Tao; Wuhan University. Renmin Hospital. Department of Organ Transplantation. Wuhan. CN
  • Zhang, Lu; Wuhan University. Renmin Hospital. Department of Organ Transplantation. Wuhan. CN
  • Chen, Zhong-bao; Wuhan University. Renmin Hospital. Department of Organ Transplantation. Wuhan. CN
  • Wang, Zhi-shun; Wuhan University. Renmin Hospital. Department of Organ Transplantation. Wuhan. CN
  • Ma, Xiao-xiong; Wuhan University. Renmin Hospital. Department of Organ Transplantation. Wuhan. CN
  • Li, Dongyu; Wuhan University. Renmin Hospital. Department of Organ Transplantation. Wuhan. CN
Acta cir. bras ; Acta cir. bras;31(3): 176-182, Mar. 2016. graf
Article in En | LILACS | ID: lil-777094
Responsible library: BR1.1
ABSTRACT
ABSTRACT

PURPOSE:

To investigate the potential effects of pretreatment with allopurinol on renal ischemia/reperfusion injury (IRI) in a rat model.

METHODS:

Twenty four rats were subjected to right kidney uninephrectomy were randomly distributed into the following three groups (n=8) Group A (sham-operated group); Group B (ischemic group) with 30 min of renal ischemia after surgery; and Group C (allopurinol + ischemia group) pretreated with allopurinol at 50 mg/kg for 14 days. At 72 h after renal reperfusion, the kidney was harvested to assess inflammation and apoptosis.

RESULTS:

Pretreatment with allopurinol significantly improved renal functional and histological grade scores following I/R injury (p<0.05). Compared with Group B, the expression levels of caspase-3 and Bax were markedly reduced in Group C, meanwhile, whereas expression of bcl-2 was clearly increased (p<0.05). A newly described marker of inflammation, High Mobility Group Box 1(HMGB1), showed reduced expression in Group C (p<0.05).

CONCLUSION:

Pretreatment with allopurinol had a protective effect on kidney ischemia/reperfusion injury, which might be related to the inhibition of HMGB1 expression.
Subject(s)
Key words

Full text: 1 Index: LILACS Main subject: Reperfusion Injury / Allopurinol / Ischemic Preconditioning / Protective Agents / HMGB1 Protein / Kidney Type of study: Prognostic_studies Limits: Animals Language: En Journal: Acta cir. bras Journal subject: Cirurgia Geral / Procedimentos Cir£rgicos Operat¢rios Year: 2016 Type: Article

Full text: 1 Index: LILACS Main subject: Reperfusion Injury / Allopurinol / Ischemic Preconditioning / Protective Agents / HMGB1 Protein / Kidney Type of study: Prognostic_studies Limits: Animals Language: En Journal: Acta cir. bras Journal subject: Cirurgia Geral / Procedimentos Cir£rgicos Operat¢rios Year: 2016 Type: Article