Aloe vera na criopreservação do sêmen de tambaqui (Colossoma macropomum) / Aloe vera in the cryopreservation of tambaqui (Colossoma macropomum) sperm
Arq. bras. med. vet. zootec
; 67(3): 945-949, May-Jun/2015. tab
Article
in Pt
| LILACS
| ID: lil-779232
Responsible library:
BR68.1
ABSTRACT
This study aimed to evaluate the extract of Aloe vera (AV) associated or not with 10% Dimethylsulfoxide (DMSO) in cryopreservation of tambaqui semen. For the formation of the pools (n= 14), 30 males were hormonally induced twice. Each pool had the objective motility, curvilinear velocity, straight-line velocity, average path velocity and morphology analyzed before and after cryopreservation of semen. The means for cryopreservation were constituted of Powder Coconut Water-104 diluent added DMSO and/or AV (5 or 10%). After cryopreservation, motility, velocities and morphology were reduced significantly when compared to fresh semen. For sperm motility the best treatment was that using only DMSO (20,86±8,31) and DMSO + 5% AV (15.71±9.77). For the velocities, the worse treatment was DMSO+10% AV. Treatment with only the addition of DMSO had a significantly higher effect than others on percentage of morphologically normal sperm. The mean correlation found was between motilityand the rate of morphologically normal sperm (r = 0.687). In conclusion, the addition of AV does not provide greater protection for spermatozoa during cryopreservation.
Key words
Full text:
1
Index:
LILACS
Main subject:
Semen Preservation
/
Cryoprotective Agents
/
Aloe
/
Characiformes
Limits:
Animals
Language:
Pt
Journal:
Arq. bras. med. vet. zootec
Journal subject:
MEDICINA VETERINARIA
Year:
2015
Type:
Article