Chemotactic effect of PAF-acether on peritoneal eosinophils from normal rats
Braz. j. med. biol. res
; 22(9): 1151-4, 1989. ilus
Article
in En
| LILACS
| ID: lil-83193
Responsible library:
BR26.1
ABSTRACT
The chemotactic activity of PAF-acether was compared with that of tetrapeptide eosinophil chemotactic factors of anaphylaxis (ECF-A, Ala-Gly-Ser Glu and Val-Gly-Ser-Glu) using eosinophils obtained from the peritoneal cavity of normal rats. Cells were isolated by separation over discontinuous metrizamide gradients which resulted in eosinophil suspensions of 80 to 90% purity. PAF-acether produced 7-fold greater than the maximal activity obtained with the ECF-A-tetrapeptides. BN 52021 and WEB 2086 inhibited PAF-acether-induced eosinophil chemotaxis in a dose-dependent manner, suggesting that this phenomenon is mediated by specific PAF-acether receptors
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Index:
LILACS
Main subject:
Peritoneal Cavity
/
In Vitro Techniques
/
Platelet Activating Factor
/
Chemotactic Factors, Eosinophil
/
Eosinophils
Limits:
Animals
Language:
En
Journal:
Braz. j. med. biol. res
Journal subject:
BIOLOGIA
/
MEDICINA
Year:
1989
Type:
Article
/
Congress and conference