The dot-ELISA test for the detection of anti-PGL-1 IgM in leprosy patients and their contacts
Braz. j. med. biol. res
; 24(5): 441-8, 1991. tab
Article
in En
| LILACS
| ID: lil-99475
Responsible library:
BR26.1
RESUMO
Since dot-ELISA has recently been reported to be a sensitive, simple and method, we have compared it with the conventional microplate ELISA method. Sera of 124 leprosy patients, 136 household and professional contacts, and 92 controls were tested for a antibodies against a Mycobacterium leprae antigen using dot-ELISA on nitrocellulose membrane filters and microplate ELISA. The sensitive of the techniques was similar for multibacillary patients, but dot-ELISA was less sensitive for paucibacillary patients although it was more specific (100%) than ELISA (93,4%). Of 21 household contacts that gave a response by ELISA, 3 were also positive by dot-ELISA; one of these 3 developed indeterminate leprosy 12 months later and the other was diagnosed as borderline lepromatous after 28 months. These data indicate that dot-ELISA has a high spedificity and can be a useful tool in field evaluation
Search on Google
Index:
LILACS
Main subject:
Immunoglobulin M
/
Enzyme-Linked Immunosorbent Assay
/
Glycolipids
/
Immunoblotting
/
Leprosy
/
Antigens, Bacterial
Type of study:
Diagnostic_studies
/
Prognostic_studies
Limits:
Humans
Language:
En
Journal:
Braz. j. med. biol. res
Journal subject:
BIOLOGIA
/
MEDICINA
Year:
1991
Type:
Article