Agrobacterium-mediated transformation of chickpea with alpha-amylase inhibitor gene for insect resistance.
J Biosci
; 2006 Sep; 31(3): 339-45
Article
in En
| IMSEAR
| ID: sea-110904
ABSTRACT
Chickpea is the world's third most important pulse crop and India produces 75% of the world's supply. Chickpea seeds are attacked by Callosobruchus maculatus and C. chinensis which cause extensive damage. The alpha-amylase inhibitor gene isolated from Phaseolus vulgaris seeds was introduced into chickpea cultivar K850 through Agrobacterium-mediated transformation. A total of 288 kanamycin resistant plants were regenerated. Only 0.3% of these were true transformants. Polymerase chain reaction (PCR) analysis and Southern hybridization confirmed the presence of 4.9 kb alpha-amylase inhibitor gene in the transformed plants. Western blot confirmed the presence of alpha-amylase inhibitor protein. The results of bioassay study revealed a significant reduction in the survival rate of bruchid weevil C. maculatus reared on transgenic chickpea seeds. All the transgenic plants exhibited a segregation ratio of 31.
Full text:
1
Index:
IMSEAR
Main subject:
Phytohemagglutinins
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Rhizobium
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Seeds
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Transformation, Genetic
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Protein Engineering
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Promoter Regions, Genetic
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Plants, Genetically Modified
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Cicer
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Plant Lectins
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Weevils
Language:
En
Journal:
J Biosci
Year:
2006
Type:
Article