Effective method for extraction of larvicidal component from leaves of Azadirachta indica and Artemisia annua Linn.
J Environ Biol
; 2006 Jan; 27(1): 103-5
Article
in En
| IMSEAR
| ID: sea-113279
ABSTRACT
Leaves of Artemisia annua linn. and Azadirachta indica were extracted in petroleum ether and hexane respectively by different methods of extraction i.e. cold extraction, reflux extraction and soxhlet extraction. The crude extract obtained was tested against third instar larvae of Anopheles stephensi. On comparison of larval mortality of crude extract obtained by these three methods, both soxhlet and reflux extraction method showed 100% mortality at 200 ppm after 48 hr in case of A. annua. However LC50 (20 ppm) value of crude extract obtained by soxhlet extraction showed better results than reflux extraction (35 ppm) method after 72 hr. In case of A. indica, crude obtained by soxhlet showed 100% mortality (after 48 hr) at 250 ppm and LC50 of 69 ppm at 72 hr. Reflux extraction does not show any appreciable mortality even at 250 ppm concentration.
Full text:
1
Index:
IMSEAR
Main subject:
Plant Extracts
/
Plant Leaves
/
Artemisia annua
/
Azadirachta
/
Insecticides
/
Larva
/
Animals
/
Anopheles
Type of study:
Evaluation_studies
Language:
En
Journal:
J Environ Biol
Year:
2006
Type:
Article