Use of sodium transfer tissue biosensor (STTB) for monitoring of marine toxic organism.
J Environ Biol
;
2004 Oct; 25(4): 431-6
Article
in English
| IMSEAR
| ID: sea-113350
ABSTRACT
A highly sensitive sodium (Na+) transfer tissue biosensor (STTB) was designed using a frog bladder membrane to measure paralytic shellfish poisons (PSP). The STTB consists, of a Na+ electrode covered by the membrane, which was then integrated into a flow-through system for continuous measurements. In the absence of Na+ channel blocker, active transfer of Na+ occurred from inside to outside across the frog membrane. When the STTB was used to measure the Na+ -dependent dissociation of PSP, it was able to detect PSB at a level contained in a single cell. However, 5 fg or higher (100 cells or more) is needed for accurate and reproducible measurements. The toxicity obtained by the STTB was significantly correlated (r = 0.9449) to that determined by the HPLC. Therefore, the simple method of the STTB can be used not only to detect a low level PSP in toxic plankton populations, but also to monitor poisons in shellfish.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Anura
/
Saxitoxin
/
Sodium
/
Tetrodotoxin
/
Biological Transport, Active
/
Urinary Bladder
/
Dinoflagellida
/
Biosensing Techniques
/
Environmental Monitoring
/
Chromatography, High Pressure Liquid
Language:
English
Journal:
J Environ Biol
Year:
2004
Type:
Article
Similar
MEDLINE
...
LILACS
LIS